FastGene Block & Go

Western blocking solution and signal enhancer

2-in-1 solution: Blocking reagent and signal enhancer
3-in-1 step procedure: Blocking of the membrane + primary antibody + secondary antibody in one step
Time-saving: Up to 2.5 h less hands-on-time compared to conventional protocol using milk or BSA
Increased detection sensitivity: Detection of proteins from up to 0.5 µg lysate
Flexible use: Usable in single step or two step protocols
Protein-free: Reduced overall background and minimization of non-specific signals

Cat. No. FG-CH05 500 mL

FastGene Block & Go - 500 mL bottle (FG-CH05)

FastGene Block & Go - 500 mL bottle with package (FG-CH05)

Details

FastGene^® Block & Go serves a blocking solution as well as signal enhancer in Western blot analysis.
It provides blocking and primary and secondary antibody hybridization in one step. Furthermore, it enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. FastGene^® Block & Go reduces time and labor-intensive Western bot procedure.

Features

3 steps in one: blocking of the membrane, primary and secondary antibody in one step
Enhanced antibody signal: 2-5-fold increase in signal intensity for most protein targets.
Enhance time-saving: It saves at least 2 hours in the antibody detection process during the Western Blot, with only one hour needed.
Effective with any ECL: After the antibody detection process, the signal can be developed with both HRP (horseradish peroxidase) and AP (alkaline phosphatase) substrates.
Less hands-on steps: No 3 wash steps are required, meaning no need to transfer the membrane in&out of the container.
Compatible with PVDF & NC membrane: Regardless of the pore size, ‘Block & Go’ minimizes the background from non-specific protein binding by antibodies.
Protein free: Reduces overall background and minimizes non-specific signals often seen with ECL detection.

Time saving

By combining 3 steps in one step (blocking + primary antibody + secondary antibody), the procedure can be reduced about 1 h (2-step procedure) or even 2.5 h (1-step procedure) compared to conventional protocol using milk or BSA.

FastGene Block&Go - time saving