Details
MIDORIGreen Xtra – Non-carcinogenic DNA stain of the latest generation – Ultra-sensitive for LED illumination
MIDORIGreen Xtra is a new highly sensitive green fluorescent stain for a safe visualization of DNA and RNA in agarose gels. This DNA stain is a safe and better alternative to the traditional nucleic acid stain ethidium bromide (EtBr). Remarkably, agarose gels stained with MIDORIGreen Xtra have a very low background fluorescence, which makes the identification of low amounts of DNA very easy. Be one of the first and test MIDORIGreen Xtra for a safe detection of DNA with an unbeatable sensitivity.
Ultra-high sensitivity of DNA bands detected with MIDORI Green Xtra. Left: Detection with a Blue/Green LED transilluminator. Right: Detection with a Blue LED transilluminator.
Ultra-sensitive DNA signals and low background – Just a perfect signal
MIDORIGreen Xtra is optimized for Blue/Green and Blue LED light, leading to unbeatable fluorescence signals of DNA and RNA in agarose gels. In addition, UV-light is also suitable for the detection of nucleic acid, but less efficient than non-damaging visible light. Remarkably, MIDORIGreen Xtra did not stain the agarose gel, leading to an excellent signal to noise ratio.
Safe Alternative to Ethidium Bromide
MIDORIGreen Xtra delivers even better DNA/RNA signals than ethidium bromide. However, this innovative DNA stain is non-carcinogenic, non-mutagenic and non-toxic and therefore not harmful for your health. Independent laboratories confirmed its safety: Both the mutagenicity test (ames test) and the cytotoxicity test were negative. Convince yourself and download here our safety report.
Cynthia Geelen, KNAW, Netherlands Institute for Neuroscience –
I am amazed at how well Midori Green performs compared to Ethidium Bromide, which I have been using throughout my working career. The comparable results have made it an easy choice for me to switch to create a safer workplace.
Verified User
Diagnostik Labor, Hamburg –
Sehr schön zu sehen ist, dass der Xtra-Farbstoff deutlich besser in der Sensitivität und im fast nicht gefärbten Hintergrund ist. Neben den Markers ist BSA aufgetragen, um zu sehen, ob wir hier auch Proteine aus z.B. Restriktionsverdauen färben. Aber auch das ist sehr positiv, da die Proteine nicht gefärbt werden. Wir haben uns auch schon den Midori Green Xtra-Farbstoff bei Ihnen bestellt und wechseln jetzt dauerhaft auf diesen.
Translation by NIPPON Genetics EUROPE:
It is very nice to see that the Xtra dye is significantly better in sensitivity and in the almost non-colored background. In addition to the markers, BSA is applied to see whether we are also staining proteins from e.g. restriction digests. But this is also very positive because the proteins are not colored. We have already ordered the Midori Green Xtra dye from you and are now permanently switching to it.
Verified User
Researcher at University of Münster (WWU), Germany –
A very safe and sensitive dye. Works extremely good with the blue/green LED. Havent tested it on a blue table, but sensitivity is much better then other ethidiumbromide alternatives we tested. It even works extremely well with light on in the lab using the amber shield or googles to extract the bands from the gel. We are completely satisfied and convinced.
Verified User
Matt from Nextbiotics –
Midori Green Xtra shows fantastic performance in the lab and is half the price per gel of SYBR Safe, so we’ll be switching to this awesome dye for all of our agarose gels moving forward!
Verified User
IPK Gatersleben, Plant Reproductive Biology –
This stain is the first real replacement for ethidiumbromide. So I´m satisfied.
Verified User
Teacher from private high school in Japan –
In class experiments, it is important to have all three results of “Successful experiment”, “Easy to get the result” and “Easy handling (safe)” at the same time. In that respect, MIDORI Green Xtra is significantly superior to other products.
Perfectly fit to FAS-Nano’s Blue/Green LED, and the band was clearly visible. With this instrument, you can take an image with iPad and see the results immediately while giving an explanation of electrophoresis result to my students. Blue LED also worked with this dye by the way.
Verified User
Japanese Researcher from National Cancer Research Center –
Compared to the Etidium Bromide, the sensitivity is absolutely fine with having better safety, and also easier handling than the Midori Green Direct. I really like this dye because it can be used with both In-gel staining and post-staining.
Verified User
Japanese Researcher, Jichi Medical University Department of Regenerative Medicine, Shimotsuke, Japan –
Overwhelming results with Blue LED light. Much better than Ethidium bromide!
Verified User