MIDORI Green Easy

Details

MIDORI^Green Easy – Non-carcinogenic DNA stain of the latest generation – Ultra-sensitive for LED illumination

• MIDORI^Green Easy is the new member of the MIDORI^Green family and a highly sensitive green fluorescent stain for a safe visualization of DNA and RNA in agarose gels.
• MIDORI^Green Easy (MG12) comes in a volume of 0.4 mL and a concentration of 10,000 x (for staining 4 L of agarose).
• This DNA stain is a safe and better alternative to the traditional nucleic acid stain ethidium bromide (EtBr).
• Remarkably, agarose gels stained with MIDORI^Green Easy have a very low background fluorescence, which makes the identification of low amounts of DNA very easy.

Excellent signal quality

We developed MIDORI^Green Easy to have the same excellent signal quality and low background as MIDORI^Green Xtra. It is also unbeatable with visible excitation light and shows the best results with our Blue/Green LED technology. The bands show outstanding sharpness and sensitivity. MIDORI^Green Easy was designed to make the switch from SYBR^® Safe as convenient as possible for you and get your DNA signals to the next level. If you are convinced by the quality of our MIDORI^Green Easy you can also try the more concentrated MIDORI^Green Xtra and adapt your staining protocol accordingly.

Figure 1: MIDORI^Green Easy shows the same ultra-sensitive and sharp DNA bands as MIDORI^Green Xtra when using Blue/Green LED excitation light. 

Safe alternative to ethidiumbromide

MIDORI^Green  Easy delivers even better DNA/RNA signals than ethidium bromide. However, this innovative DNA stain is non-carcinogenic, non-mutagenic and non-toxic and therefore not harmful for your health. Independent laboratories confirmed its safety: Both the mutagenicity test (ames test) and the cytotoxicity test were negative.

Easy switch from SYBR^® Safe

MIDORI^Green Easy enables an effortless switch from using SYBR^® Safe to stain agarose gels. MIDORI^Green Easy has exactly the same dye concentration (10,000 x) and tube volume (0.4 mL) as SYBR^® Safe (sufficient for 4 L agarose) – for less than half the price.

The performance of MIDORI^Green Easy and SYBR Safe is the same, depart from the difference that heating of the DNA stain is recommended for SYBR Safe but MIDORI^Green Easy should be added to the agarose after heating.

Figure 2: Similar results where shown when using MIDORI^Green Easy and SYBR Safe with three different DNA markers (MWD50, MWD100 and MWD1P).
The procedures were performed according to the related product’s protocol (A and B) and differing from the related product’s protocol (C and D).
5 µL SYBR Safe or 5 µL MIDORI^Green Easy were added to 50 mL agarose-TAE (1.5% agarose-TAE, 100 V, ~ 30 min).
DNA Marker: MWD50 (FastGene^® 50 bp DNA Marker), MWD100 (FastGene^® 100 bp DNA Marker), MWD1P (FastGene^® 1 kb bp DNA Marker Plus)

Figure 3: Similar results where shown when using MIDORIGreen Easy and SYBR Safe for a DNA dilution series (50-500 ng).
The procedures were performed according to the related product’s protocol (A and B) and differing from the related product’s protocol (C and D).
5 µL SYBR Safe or 5 µL MIDORIGreen Easy were added to 50 mL agarose-TAE (1.5% agarose-TAE, 100 V, ~ 30 min).
DNA Marker: MWD1P (FastGene® 1 kb bp DNA Marker Plus)