Xho I

Restriction Enzyme

Recognition Sequence	Amount
C/TCGGG GAGCT/C	5000 Units, 20 Units/µL

Overhang	CpG Methylation
5′ TCGA	Conditionally sensitive

Incubation Temp.	Heat Inactivation
37°C	80°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
50%	100%	100%	100%	100%

Supplied Buffer	Enzyme Type
Buffer IV	Type IIP

Isoschizomers
PaeR7I, Sfr274I, SlaI

Cat. No. FG-XhoI

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