TspM I

Restriction Enzyme

Recognition Sequence	Amount
C/CCGGG GGGCC/C	200 Units, 5 Units/µL

Overhang	CpG Methylation
5′ CCGG	Sensitive

Incubation Temp.	Heat Inactivation
75°C	No

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
50%	75%	50%	100%	100%

Supplied Buffer	Enzyme Type
Buffer IV	Type IIP

Isoschizomers
Cfr9I, SmaI*, XmaI

Cat. No. FG-TspMI

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