Spe I

Restriction Enzyme

Recognition Sequence	Amount
A/CTAGT TGATC/A	500 Units, 10 Units/µL

Overhang	CpG Methylation
5′ CTAG	Not sensitive

Incubation Temp.	Heat Inactivation
37°C	80°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
50%	100%	75%	100%	100%

Supplied Buffer	Enzyme Type
Buffer IV	Type IIP

Isoschizomers
AhlI, BcuI

Cat. No. FG-SpeI

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