Sma I

Restriction Enzyme

Recognition Sequence	Amount
CCC/GGG GGG/CCC	2000 Units, 20 Units/µL

Overhang	CpG Methylation
Blunt	Sensitive

Incubation Temp.	Heat Inactivation
25°C	65°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
0%	0%	0%	100%	100%

Supplied Buffer	Enzyme Type
Buffer IV	Type IIP

Isoschizomers
Cfr9I, TspMI, XmaI*

Cat. No. FG-SmaI

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