Nhe I

Restriction Enzyme

Restriction Sequence	Amount
G/CTAGC CGATC/G	1000 Units, 10 Units/µL

Overhang	CpG Methylation
5′ CTAG	Conditionally sensitive

Incubation Temp.	Heat Inactivation
37°C	65°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
100%	100%	10%	100%	100%

Supplied Buffer	Enzyme Type
Buffer II	Type IIP

Isoschizomers
AsuNHI, BmtI, BspOI

Cat. No. FG-NheI

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