Hph I

Restriction Enzyme

Recognition Sequence	Amount
GGTGANNNNNNNN/ CCACTNNNNNNN/N	1000 Units, 5 Units/µL

Overhang	CpG Methylation
3′ N	Not sensitive

Incubation Temp.	Heat Inactivation
37°C	65°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
100%	75%	10%	100%	100%

Supplied Buffer	Enzyme Type
Buffer IV	Type IIS

Isoschizomer
AsuHPI

Cat. No. FG-HphI

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