Fok I

Restriction Enzyme

Recognition Sequence	Amount
GGATGNNNNNNNNN/NNNN CCTACNNNNNNNNNNNNN/	1000 Units, 5 Units/µL

Overhang	CpG Methylation
5′ NNNN	Sensitive

Incubation Temp.	Heat Inactivation
37°C	65°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
100%	100%	10%	100%	100%

Supplied Buffer	Enzyme Type
Buffer IV	Type IIS

Isoschizomers
BseGI, BstF5I, BtsCI*

Cat. No. FG-FokI

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