BspE I

Restriction Enzyme

Recognition Sequence	Amount
T/CCGGA AGGCC/T	1000 Units, 10 Units/µL

Overhang	CpG Methylation
5′ CCGG	Conditionally sensitive

Incubation Temp.	Heat Inactivation
37°C	80°C for 20 min

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
10%	10%	100%	10%	100%

Supplied Buffer	Enzyme Type
Buffer III	Type IIP

Isoschizomers
AccIII, Aor13HI, BseAI, Bsp13I, Kpn2I, MroI

Cat. No. FG-BspEI

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