Bcl I

Restriction Enzyme

Recognition Sequence	Amount
T/GATCA ACTAG/T	3000 Units, 10 Units/µL

Overhang	CpG Methylation
5′ GATC	Not sensitive

Incubation Temp.	Heat Inactivation
50°C	No

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
50%	100%	100%	75%	100%

Supplied Buffer	Enzyme Type
Buffer III	Type IIP

Isoschizomers
FbaI, Ksp22I

Cat. No. FG-BclI

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