ApaL I

Restriction Enzyme

Recognition Sequence	Amount
G/TGCAC CACGT/G	2500 Units, 10 Units/µL

Overhang	CpG Methylation
5′ TGCA	Conditionally sensitive

Incubation Temp.	Heat Inactivation
37°C	No

Activity in FastGene Buffer

Buffer I	Buffer II	Buffer III	Buffer IV	FastCut Buffer
50%	100%	50%	100%	100%

Supplied Buffer	Enzyme Type
Buffer IV	Type IIP

Isoschizomers
Alw44I, VneI

Cat. No. FG-ApaLI

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