FastGene Kickspeed 2x DNA Ligation Mix

Ligation of DNA strands

  • Ready-to-use solution for 50 reactions
  • Quick ligation of DNA fragments in 5 min at room temperature
  • Catalyses the formation of a phosphodiester bond for the connection of two DNA strands
  • T4 Ligase will join blunt ends and sticky ends
  • Supplied with optimal buffer conditions for an efficient ligation
  • Highest activity and purity

 

Price on request


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Cat. No. FG-LM50 50 rxns
Ligases

Details

Kickspeed 2x DNA Ligation Mix

The FastGene® Kickspeed 2X DNA Ligation Mix is a ready-to-use solution of T4 DNA Ligase for both sticky-end and blunt-end ligation reactions. This master mix enables quick ligation in a short incubation time (< 5min) at room temperature.

Characteristics

  • 2X Master mix of T4 DNA Ligase and ligation enhancer
  • Fastest ligation of dsDNA with sticky end and blunt end
  • Incubation-free ligation

Applications

  • Vector construction
  • Linker ligation
  • Fragment assembly
  • Routine cloning
T4 DNA Ligase

The FastGene® T4 DNA Ligase catalyzes the formation of a covalent bond between the 5′-phosphate and 3′-OH in nicked duplex DNA or at two DNA ends. This activity is very useful to ligate DNA fragments with either cohesive or blunt ends, that are generated by restriction enzyme digestion. T4 DNA Ligase can also ligate RNA with DNA or RNA in a double helix with low efficiency. The T4 DNA Ligase is cloned and expressed in E. coli, and purified to homogeneity. The Ligase is free of endonuclease, exonuclease and phosphatase.

Downloads

Manual

Manual | FastGene Kickspeed 2x DNA Ligation Mix | FG-LM50

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