Details
Bambanker™ Direct for hybridomas
There are certain cell types, such as hybridomas, which exhibit increased sensitivity to “over” manipulations. These cells often exhibit higher death rates or unwanted differentiation after long-term storage and freeze thaw cycling. It is for these types of cells that Bambanker™ Direct was created – to provide tight environmental control. By eliminating the centrifugation step, cells can now be frozen for long-term storage immediately upon addition of the novel cryoprotectant. Just add Bambanker™ Direct, one-toone, with your cells in media and directly place in freezer. And like with the original Bambanker™, the Direct version has no serum, so it is great for cells which animal-derived serum may be an issue.
Procedure for the cryopreservation of cells
GMP quality
All products of the Bambanker™ series are manufactured in facilities compliant with current good manufacturing practices (GMP).
The products are subject to a solid quality management system that ensures the consistency, reliability and high quality you can rely on.
Notification: Manufacturer’s notification for Bambanker as GMP-manufactured product (pdf file)
Flexible
There a four different versions of Bambanker™.
- All Bambanker™ types are free of fetal bovine serum (FBS) and have a defined and constant composition.
- They also do not contain antibiotic or antifungal ingredients.
The four different types of Bambanker™ are recommended depending on your specific needs:
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Bambanker™ (standard for all cell lines, package units: 20 mL, 5 x 20 mL, 100 mL) Please note that the 120 mL bottle size (BB01) is no longer available and has been replaced by the new 100 mL bottle size (BB05). |
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Bambanker™ Direct (for hybridoma cells, package unit: 20 mL) |
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Bambanker™ hRM (for ES and iPS cells, package units: 20 mL, 100 mL) Please note that the 120 mL bottle size (BBH03) is no longer available and has been replaced by the new 100 mL bottle size (BBH04) |
D. Iliev, Institute of Molecular Biology, Bulgarian Academy of Sciences, Bulgaria –
We have had an excellent experience with Bambanker Direct! The freezing protocol is very easy and straightforward and allows us to quickly process large numbers of samples. In our protocol, we grow the single cell clones in 24 well plates. Upon reaching confluency, we trypsinize and we use 2/3 of the suspension for genotyping lysates and 1/3 of the suspension (50-70 000 cells) is mixed with an equal volume of Bambanker Direct (100 ul) and stored at -80. So far all of the clones we have thawed and recultured have recovered very quickly. We have used clones kept frozen in Bambanker for several months and their viability has been excellent! Overall, this product gets my best recommendations!
Verified User
Researcher from Japan –
I am using Bambanker Direct for frozen hybridomas when making monoclonal antibodies.
After screening, the culture of positive cells was scaled from 96 wells to 24 wells. Pipette 250 μl of cells in the growth phase from the centre where the 24 well cells are dense, mix it with an equal amount of Bambanker Direct, and freeze. Freezing can be done quite easily without the need to centrifuge the cells and so on. I think that it will be powerful for freezing floating cells. Bambanker is used for adhesion cells and Bambanker Direct is used for floating cells.
Verified User
Mady , Tübingen University –
We had a major liquid Nitrogen tank issue. We looked for an alternative. We requested a free sample to try.
Bambanker was sooo easy to use and very good on sensitive cells (no toxicity see), which can also be stored at -80. No more DMSO/FBS!!
I highly recommend it (10 years+ (primary& cell line) cell culture experience!)
Verified User