IC Green qPCR
Quantification of Gene Expression
Quantification of Gene Copy number
Detection of Gene Expression (Knock-out analysis)
It is well-known that SYBR green is extensively inhibiting the qPCR. This fact led to the development of SYBR resistant enzymes. An alternative approach is to develop a dye that does not inhibit the reaction. This dye is named FastGene® IC Green. FastGene® IC Green is an intercalating dye, only detecting double stranded DNA. By not inhibiting the reaction, the FastGene® IC Green enhances the processitivity enabling the detection of genes at a lower CT-value. The superior buffer chemistry enables the detection of low copy number genes, which with other dyes could not be detected. The comparison to competitors shows that FastGene® IC Green is one of the best qPCR mixes available. This has been confirmed by customers analyzing many different genes.
The FastGene® IC Green buffers were designed to have a superior robustness. This guarantees the linearity of the qPCR and creates a better accuracy, essential for reproducible results. Additionally, qPCRs can be performed at lower amplifcation times for example using fast protocols.
The FastGene® IC Green kit is universal. The reference dyes come in a separate vial and can be added once to the master mixes. Hence, this kit can be used with qPCR instruments which need a high ROX™ concentration as well as instruments that need a low concentration or no ROX™. There is even a special version with fluorescein.
Fig.1: Comparison of FastGene® IC Green (black & red) with the market leading competitors KB (green) and T (blue). The differences of the CT-values were under 1 cycle.
Fig. 2: Comparison of FastGene® IC Green (blue) with the market leading competitors KB (yellow). The differences of the CT-values were under 1 cycle. Data was kindly provided by an independent researcher.
|LS4001||FastGene® 2x IC Green Universal (ROX™)||100 Reactions|
|LS4005||FastGene® 2x IC Green Universal (ROX™)||500 Reactions|
|LS4050||FastGene® 2x IC Green Universal (ROX™)||5000 Reactions|
|LS4101||FastGene® 2x IC Green Universal (Fluorescein)||100 Reactions|
|LS4105||FastGene® 2x IC Green Universal (Fluorescein)||500 Reactions|
|LS4150||FastGene® 2x IC Green Universal (Fluorescein)||5000 Reactions|
|Manual||Technical Data Sheet of the FastGene IC Green||Download|