Nippon Genetics is proud to introduce a novel reagent specially formulated in order to eliminate nucleic acid purification for PCR analysis. DNAreleasy Advance is a continuous development of our well know lysis reagent DNAreleasy suitable for template preparation of PCR experiments. DNAreleasy Advance replaces time consuming and tedious extractionand purification methods. The released DNA can be usde directly in a PCR reaction or can be stored at -20°C for several months. So far DNAreleasy has been tested on various cell types, including:
Genomic DNA scallops was isolated by DNAreleasy Advance and a part of the supernatant was directly added to the PCR reaction. The agarose gel shows the high yield of amplified DNA fragment.
The same amplified DNA was used for subsequent sequencing experiments.
Total DNA was isolated by DNAreleasy Advance from various plant species. Thereafter the melting curve was determined by RT PCR by using a Light Cycler instrument (Roche):
Genomic DNA was isolatedby using DNAreleasy Advance from different materials and organism and analyzed by Roche's Light Cycler analysiert (Cytb primer):
The second-generation KAPA2G Robust DNA Polymerase was evolved to solve - achieve consistent amplification across a broad range of amplicon types (both GC and AT-rich). The versatility and robustness of the polymerase allows for consolidation of PCR cycling protocols and reaction conditions while increasing success rates. The high performance of the KAPA2G Robust DNA Polymerase eliminates the need for multiple enzymes and protocols - standardize your PCRs with a single enzyme solution.
|LS05||DNAreleasy Advance||300 µl for 10 preps|
|LS06||DNAreleasy Advance||1500 µl for 50 preps|
|LS08||Fastgene® Direct PCR Kit||50 preps / 100 rxns|
|LS09||Fastgene® Direct PCR Kit||50 preps / 200 rxns|
|LS10||Fastgene® Direct PCR Kit||10 preps / 100 rxns|
|LS12||FastGene® Direct High Speed PCR kit||50 preps / 100 rxns|
|LS13||FastGene® Direct High Speed PCR kit||50 preps / 200 rxns|
|LS14||FastGene® Direct High Speed PCR kit||10 preps / 100 rxns|