Nippon Genetics Europe is able to offer 2 different protein marker, one with pink/blue bands and the other with a combination of blue and red bands. We are recommending to use the PINK protein marker for all kinds of protein gels, whereas the blue/red combination is preferably used for standard Tris/Glycine gels.

Cat. No. MWP02 Size: 2 x 500µl

Broad range: 15 - 175 kDa.
Ready to use: Supplied in a loading buffer for direct loading on gels.
Easy to identify: Includes the 40 and 90 kDa reference bands coupled with a blue dye.
Sharp bands

Description

The PiNK Prestained Protein Ladder contains 10 proteins that resolve
into sharp, tight bands in the range of 15-175 kDa. The PiNK Prestained
Protein Ladder allows you to monitor molecular weight separation
during electrophoresis, estimate molecular weights of proteins of interest,
and evaluate western transfer efficiency.

Applications

• Monitoring of protein migration during SDS-polyacrylamide gel
electrophoresis.
• Monitoring of protein transfer onto membranes during Western
blotting.
• Sizing of proteins on SDS-polyacrylamide gels and Western blots.

Quality Control

Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.

Storage Buffer

53 mM Tris-phosphate pH 7.5 (at 25°C ), 2.7 % SDS, 2 mM
2-Mercaptoethanol, 3.6 M Urea, and 23 % (v/v) Glycerol.
(Approximately 0.1~0.2 mg/ml of each protein in the buffer)

Storage

Store at -20°C for 12 months
Stable at +4°C for 3 months

Recommendations for Loading

1.Thaw the ladder either at room temperature or at 37-40°C for a few
   minutes to dissolve precipitated solids. Do not boil.
2.Mix thoroughly to ensure the solution is homogeneous.
3.Load the following volumes of the ladder on SDS-polyacrylamide gel:
   - 10 μl per well for mini-gels, 5 μl per well for blots
   - 20 μl per well for large gels, 10 μl per well for blots

Cat. No. MWP01 Size: 500µl

Broad range: 15 - 175 kDa.
Ready to use: Supplied in a loading buffer for direct loading on gels.
Easy to identify: Includes the 20 and 75 kDa reference bands coupled with an orange dye.
Sharp bands

Description

The Prestained Protein Ladder is a two color protein standard with 10 prestained proteins covering a wide range molecular weights from 15 to 175 kDa. The ladder contains two red band at 20 and 75 kDa when separated on SDS-PAGE (Tris-glycine buffer).

The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.

The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

Applications

Quality Control

Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.

Storage Buffer

20 mM Disodium hydrogen phosphate pH 8.0 (at 25°C ), 2 % SDS,
0.2% Triton X-100,10 mM 2-Mercaptoethanol, 6 M Urea and 27%
(v/v) Glycerol.

Storage

Store at -20°C for 12 months.
Stable at +4°C for 3 months.

Recommendations for Loading

1.Thaw the ladder either at room temperature or at 37-40°C for a few
   minutes to dissolve precipitated solids. Do not boil.
2.Mix thoroughly, to ensure that the solution is homogeneous.
3.Load the following volumes of the ladder on SDS-polyacrylamide gel:
   - 5 μl per well for mini-gels, 3 μl per well for blots;
   - 10 μl per well for large gels, 6 μl per well for blots