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QPCR promotion Kapa Biosystems |
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Dear
today we would like to send you an example of a HTML newsletter that you can send to your customers in accordance to our price promotion for the QPCR kits that will be valid from now (!) until end of this year (December 31st; 2009). If you did not receive our price promotion so far please contact us and we will send you a quote today mailto:info@nippongenetics.eu.
You can pick just the pictures you like and of course you can modify this newsletter for your special needs. I hope this tool is helpful for you - all the best
Juergen
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KAPA SYBR FAST qPCR Kits for all instruments and the following features: |
| ► | Regular TAQ is inhibited by large concentration of SYBR Green. This kit contains a novel DNA polymerase engineered through a process of molecular evolution. The result is a unique enzyme, which can work with higher concentration of SYBR Green and this means: Higher sensitivity and lower CTs! |
Human B-actin (290 bp) was amplified from log-fold dilutions of human genomic DNA (20 ng to 2 pg) using KAPA SYBR® FAST qPCR Master Mix (2x) optimized for Roche LightCycler® 480 or Roche LightCycler® 480 SYBR Green I Master kit (Roche). Comparisons were performed according to the competitor protocol A: Roche protocol: 95°C, 5 min followed by 40 cycles of 95°C, 10 sec; 60°C, 15 sec; 72°C, 6 sec; or B: KAPA SYBR® FAST Protocol: 95°C 3 min followed by 40 cycles of 95°C, 10 sec; 60°C, 15 sec; 72°C, 1 sec.
For more information:www.kapabiosystems.com/products/next-generation/kapa-sybr-fast-qpcr-kits
►High speed and high performance
High speed does not compromise performance with the KAPA SYBR® FAST qPCR kits. As can been seen from the amplification plots and standard curves there is no loss in performance between fast and conventional cycling conditions even with complex targets.
| Standard cycling | Fast Cycling | |
| Activation: | 5 min. @ 95° | 3 min. @ 95°C |
| PCR Cycling | 15 s @ 95°C | 1 sec @ 95°C |
| 60 s @ 60°C | 20 s @ 60°C | |
| Cycle Time: | 40 cycles = 55 min. | 40 cycles = 14 min. |
| Elapsd Time: | 1 hr. 30 min. | 40 min. |
| RotorGene 6000HRM | 5 runs / 8 hour shift | 12 runs / 8 hour shift |
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KAPA PROBE FAST qPCR Kits designed for high throughput, fast-cycling, real-time PCR using sequence-specific fluorogenic probes and the following features: |
► Compatibility with all probe-based qPCR applications and instruments
► Fast, reproducible, and precise quantification
► Discrete clusters in SNP genotyping assays
► Multiplex qPCR
► Broad dynamic range
► Highly stable master mix for high-throughput workflows
Highly reproducible and efficient results for all 5 amplicons across a 5 point dilution series of human genomic DNA were obtained when assayed in penta-plex using a fast cycling protocol. Standard curves were generated using 4-fold dilutions of human genomic DNA (0.39 – 100 ng per reaction) tested in triplicate using the Corbett Rotor-Gene™ 6000 HRM real-time rotary analyzer. Reactions were performed in 20 μl volumes with KAPA PROBE FAST qPCR Master Mix, human genomic DNA, 200 nM of each primer and 200 nM of each hydrolysis probe (ACTB - FAM™/BHQ®-1, ERBB2 - CAL Fluor® Gold 540/ BHQ®-1, ERBB3 - CAL Fluor® Orange 560/ BHQ®-2, EGFR - CAL Fluor® Red 610/ BHQ®-2, ACTG1 - Quasar® 705/ BHQ®-2) using the following fast cycling protocol: 95 ºC for 3 min followed by 40 cycles of 95 ºC, 15 sec; 60 ºC, 15 sec.
Discrete clusters and high call rates for accurate and reproducible allelic discrimination
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All 168 human genomic DNA samples were accurately genotyped using the ABI sequence detection system (SDS) version 2.3 software (autocaller confidence level 95%). A total of 168 human genomic DNA samples were successfully genotyped along with 24 no-template controls using an ATP1B3 SNP genotyping assay on the ABI 7900HT real-time PCR system. Reactions were performed in 5 μl volumes with KAPA PROBE FAST qPCR Master Mix, human genomic DNA (10 ng per reaction), 200 nM of each primer and 200 nM of each hydrolysis probe (Allele X – FAM/ BHQ®-1, Allele Y – VIC/ BHQ®-1) using the following standard cycling protocol: 95 ºC for 10 min followed by 40 cycles of 95 ºC, 15 sec; 60 ºC, 60 sec. |
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