Newsletter

“I tested four samples with both ABI and KAPA SYBR FAST reagents. Please find attached the summary of the qPCR results. The KAPA SYBR reagent can bring down the Ct values by 1.5-2, which is amazing. I will go ahead and use it for my future CHIP experiments. Thank you!”

- Junqi Song, Duke University

Kapa HIFI and 2G Robust: This testimonial came from a huge Genome Centre in Canada

“Hi Duane, 

I just wanted to thank you for the sample of KAPAHiFi you left us. i used it for a reaction that we were having lots of trouble with due to very high secondary structure in the 80bp primer. i used your enzyme and after a little optimizing I got the correct sequence in 1 of 6 clones i screened. I screened many other clones when we used Roche high fidelity system and I couldn’t get one right clone. I was wondering if you could send me the price for this kit as well as a sample of the enzyme which you said can do 60kb per minute. 

Thanks again Andres “

Another customer form the same centre:

“Hi Duane, I tried out the Kapa2G robust kit.  We're super happy with it.  We first tested it on gDNA and the amount of template needed was very minimal, 2ng in a 25ul reaction, which is great because we often have precious gDNA samples.   We also used it to amplify directly off serial dilutions of lymphoblast cell line suspensions.  We found we were able to amplify off of between 300 and 500 cells direct.  We're very happy with that aspect as well.  I'd like to keep some of kapa2G robust around for situations where it would be useful to amplify without DNA purification or when we need to amplify off a very small amount of gDNA”.   

Dear Distributors,  I thought it worthwhile to share the attached testimonial that we received from the University of California at Berkley for KAPA HiFi primarily because it identifies the competitive products that were also evaluated.

Ron McEwan (CEO Kapa Biosystems)

Kapa HIFI

“Hi Erick,

I just wanted to share an unsolicited testimonial about the free samples of your HiFi enzyme. I had been experiencing poor yield attempting to amplify with Expand a product that should have been trivial -- a 200-bp product from a plasmid template. So, I tried all 10 PCR enzymes we have in the lab with the same primers and template and the same cycling parameters (a rather generic program, optimized for none of the enzymes) using the basic buffer conditions for each enzyme, and estimated the yield by running a portion of the reactions on a gel. 

The rankings, by yield: 

1) Invitrogen Amplitaq (low fidelity) in Roche Taq buffer (however, a non-specific band was also amplified)

2) (TIE) Pfx50 and Kapa HiFi

3) Pfu Ultra

4) Pfu Turbo

5) Phusion

6) Expand plus 5% DMSO (since it didn't work well without DMSO in previous trials)

7) Qiagen Taq (a non-specific band was also amplified) Pfu and Pfx failed to amplify any detectable product. 

For a high-fidelity application (like this test example), I'd choose Kapa HiFi. So far so good! 

Michael”

Dear Distributors, I would like to share a success story with you concerning the sale of KAPA products into Yale University in the US. Our US East Coast salesperson (Jason) has been working for 3 months to get our products included in the freezer program at Yale, which is no easy task, to do this he has to get references from existing end users to convince the stores manager to purchase our products and maintain inventory, the following communications confirm his success.

Ron McEwan (CEO Kapa Biosystems)

Hi Joe, I've known Jason since he was at Agencourt and I can say confidently that all my experiences with him have been great - he has never been a "hard sell" and relies on the quality of his products.  He handles questions and problems quickly and efficiently. KAPA enzymes are better than any we've used.  In particular, the KAPA long range enzyme out-performs four other competitors we tested. And, it's the cheapest.  I think that their standard enzymes are also of very high quality. We have uses for the Kapa LR, Fast2G, and HiFi enzymes immediately.
When we had a problem with an enzyme earlier this year, the company took our own primer set in-house and figured out what the problem was - I've never had an experience like this before.  I am excited to hear that you are considering carrying Kapa Products and I hope to purchase them from the stockroom.
Best, Keith
________________________________
Keith A. Choate MD, PhD
Instructor, Department of Dermatology
YaleUniversitySchool of Medicine

That's all for today, we have a lot of other testimonials in the pipeline, one for Kapa Blood kit from one of the most important centres in Europe and as  soon we will get the data, we will let you know. Now we would like to wish you  a good time, a lot of success and for some of you guys a nice holiday on Thursday.

Take care
Juergen

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