Newsletter :: NIPPON GENETICS EUROPE

today we would like to inform you briefly about the Next Generation Sequencing experiments. All of you guys already realized that there are new instruments in the market like the Roche 454 , the Illumina (Genome Analyzer) or the ABI Solid instrument.

Kapa Biosystems published a PDF file to show the use of their enzymes for the specific steps involved in these workflows.

This PDF file can be downloaded here next-generation-sequencing/

As you can see, there are many chances to sell Kapa products to these accounts. If you have more questions about it, don't hesitate to contact us. mailto: info@nippongenetics.de

Customer testimonial Kapa HIFI:

Hi Ron,

I have just received this reply from a customer who trialled KAPAHiFi against Phusion with great success, so I thought that I would share it with you.

“I have had the opportunity to sample the KAPAHiFi. I am extremely pleased with it too. I was trying to amplify a gene from random-primed cDNA that had proved to be impossible to amplify using normal polymerases (Pfu). I had some unsuccessful initial attempts with the normal buffer but after switching to the GC buffer and optimising the reaction according to the protocol I managed to amplify the specific product of interest.

The best thing in Kapa’s favour is that I was doing a direct comparison between KAPAHiFi and Finnzymes' Phusion Hi-Fidelity polymerase. The Phusion was unable to amplify the correct product (with optimised conditions according to the Finnzymes protocol). The only way I managed to get Phusion to amplify the specific product was when I used the PCR product from a previous KAPAHiFi reaction. So thanks again for the sample. I know I'll be going with KAPAHiFi when I need a high fidelity polymerase and also recommending it when I hear of others having troublesome PCR's”.

Kindest Regards,

Allison Hall B.Sc./Product Manager/GeneWorks Pty Ltd; AUSTRALIA

>>>> Nippon Genetics will do a HiFI promotion, so if you like to participate, please send us an email and we will send you immediately a quote!<<<<<<<<<

Customer testimonial Kapa 2G Robust for Colony PCR:

Sample Request

I am a postdoc in the lab of Prof. Jeremy Thorner in the Dept. Molecular and Cell Biology at the University of California, Berkeley.

Our experimental system is the budding yeast Saccharomyces cerevisiae, and we perform hundreds of colony PCR’s per week. We typically use the cheapest Taq we can find for this purpose, and assume that no product larger than 1 kb can be amplified. We have been disappointed with the performance of our latest, cheapest Taq purchase, from Roche, which required double the amount of enzyme to achieve reproducible colony PCR compared to the Taq from Qiagen we used previously. We would love to try a sample of your KAPA2G Taq. The ability to reproducibly amplify products larger than 1 kb would be extremely valuable to us.

Sample Response

Dear Erick,

Thank you for the sample that arrived so quickly. A student in the lab tried it yesterday to amplify a 1.6-kb product from yeast colonies and the reactions worked amazingly well. We would never have even attempted to amplify something so large with our standard colony PCR reagents. He also sequenced the products and was impressed with the fidelity of the polymerase. Would you be able to give us an idea of the cost of the kit for us here at UC Berkeley?

Thank you very much,

Michael

Take home message: Colony PCR is very popular for many customers but normally they use regular TAQ and because of the inconsistency of regular TAQ they need sometimes a much higher concentration of the enzyme Nevertheless they won't be successful if they need to generate larger products (> 1kb). Furthermore Kapa 2G Robust (and of course Robust HS) will lead to a much higher success rate, especially for difficult bacteria and/or yeast!

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