Eliminate time-consuming and expensive titrations and reduce variability in cluster density or template-to-bead ratio with a high performance qPCR solution for next-gen sequencing library quantification.

Standard methods for quantifying next-generation sequencing libraries have a number of important disadvantages. Electrophoresis and spectrophotometry measure total nucleic acid concentrations, whereas optimal cluster density or template-to-bead ratio depend on the appropriate concentration of PCR-amplifiable DNA molecules. These methods also have low sensitivity, consuming nanograms of precious samples, and are not suitable for high-throughput workflows.

Quantitative PCR (qPCR) is inherently well-suited for next-generation sequencing library quantification:

qPCR specifically quantifies only PCR-competent DNA molecules,
is highly sensitive allowing accurate quantification of low concentration libraries,
is amenable to automated liquid handling.

KAPA Library Quantification Kits are optimized for the Illumina Genome Analyzer, Roche 454 Titanium series, and Roche 454 FLX series platforms and include highly reproducible and precisely defined sets of serially diluted DNA concentration standards and state-of-the-art qPCR reagents, which contain a DNA polymerase specifically engineered for robust, SYBR^® Green I-tolerant amplification of long and difficult templates.

"Before qPCR was adopted for library quantification, cluster density was extremely variable. Implementation of the KAPA Library Quantification Kit into our sequencing workflows resulted in a significant reduction in variability across multiple libraries, negating the need for cluster amplification titration runs".

- The Broad Institute, Cambridge, MA U.S.A.

For more information, please click here www.kapabiosystems.com/products/name/kapa-library-quant-kits