The KAPA2G Robust PCR Kit is recommended for all standard end-point PCR assays, particularly those in which wild-type Taq DNA polymerase does not perform satisfactorily. The kit is based on KAPA2G Robust DNA Polymerase, a highly robust and versatile engineered enzyme that was derived from Taq DNA polymerase using the KAPA Biosystems molecular evolution platform. The novel amino acid mutations in KAPA2G Robust DNA Polymerase offer higher processivity and specific activity, which translates to robust performance across a wide range of GC- and AT-rich templates and amplicons, as well as improved tolerance to common PCR inhibitors.

Two proprietary PCR enhancers supplied with the two high-yield KAPA2G buffers offer extended optimization options for difficult amplicons or templates. Like wild-type Taq, KAPA2G Robust DNA Polymerase has 5→3 polymerase and exonuclease activities, but no 3→5 exonuclease (proofreading activity). The fidelity of KAPA2G Robust DNA Polymerase is the same as that of wild-type Taq.

Product Applications

KAPA2G Robust PCR Kits are designed for the amplification of DNA fragments up to 6 kb in standard end-point PCR assays from a wide variety of templates. It is particularly suited for:

Amplification from templates with a high GC- or AT content.
Templates containing common PCR inhibitors (e.g. salts, urea, SDS, ethanol or EDTA) at levels inhibitory to wild-type Taq.
Colony PCR.
Optimization of low yield or low specificity assays using KAPAEnhancer 1 or 2.

Amplicons generated with the KAPA2G Robust PCR Kit are suitable for routine downstream applications, including restriction enzyme digestion, cloning and sequencing.

For more information :https://www.kapabiosystems.com/product/kapa2g-robust/