Consistent amplification of a broad range of targets, covering the full spectrum of AT- and GC-rich content, using a single set of reaction conditions and one cycling protocol is the ultimate goal of any lab performing routine PCR. This concept of "Single Protocol PCR" has proven impractical due to the inherent limitations of wild-type Taq DNA Polymerase – most notably its inefficient amplification of challenging templates and narrow range of optimal PCR conditions. In order to improve PCR success rates and minimize reaction failures, many laboratories are forced to segregate difficult targets from "easy" targets and optimize individual reactions. The resulting subset of specialized assays disrupts workflows and increases turnaround time and cost by requiring a myriad of PCR reagents, reaction conditions and cycling protocols.

KAPA2G Robust DNA Polymerase is a novel, second-generation (2G) enzyme engineered for higher processivity and improved tolerance to common PCR inhibitors through a process of molecular evolution. The unique features of the enzyme supports versatile and robust amplification of a wide range of template and amplicon types. Combined with a proprietary reaction buffer that facilitates primer annealing, successful amplification of both AT- and GC-rich amplicons can finally be achieved using a simplified reaction setup and a single, fast cycling protocol that typically requires 20 – 50% less cycling time than protocols designed for wild-type DNA polymerases.

For more detailed protocol information on consolidating PCR protocols download the Single Protocol PCR Application Note here>>

Ordering and product information for KAPA2G Robust HotStart and ReadyMix here>>

Related Applications

The high processivity and improved tolerance to inhibitors of the KAPA2G Robust DNA Polymerase also translates to increased success with difficult templates, including amplicons with very high GC content (> 70%). More detailed protocol information on the use of KAPA2G Robust for routine GC-rich PCR can be found on the GC-rich applications page>>

The ability to consolidate PCR protocols and reaction conditions with KAPA2G Robust HotStart is perfect for upstream amplification prior to Sanger DNA sequencing >>