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New PCR enzymes - Kapa Biosystems

Nippon Genetics Europe GmbH is proud to represent Kapa Biosystems in several countries in Europe (for more information, please contact us info@nippongenetics.eu). Nippon Genetics is still looking for potential distributors in some countries, so please don't hesitate to contact us for further information.If you like to get more technical information, please have a look on their WEB page (www.kapabiosystems.com ) .

Expand your PCR possibilities with Kapa Biosystems next-generation DNA polymerases.  

Despite the claims of other reagents suppliers, all commercially available Taq polymerase is the same wild-type enzyme – diferences in performance are due to buffer formulation and/or enzyme concentration.  Our molecular evolution platform is capable of engineering novel polymerases that are fundamentally different at the protein  level; these engineered polymerases contain unique amino acid modifcations that confer dramatic improvements to the function of the enzyme.

 

Kapa2G Robust and Robust Hot Start:
Improve PCR success rates with this highly versatile second-generation polymerase. KAPA2G Robust DNA Polymerase offers higher processivity and specifc activity, which translates to robust performance across a wide range of GC- and AT-rich templates and amplicons, difcult samples, as well as improved tolerance to many common PCR inhibitors such as ethanol, salt, and SDS.  KAPA2G Robust HotStart is the enzyme of choice for crude samples, including colony PCR. [read more]

KAPA SYBR® FAST qPCR Kits
The frst DNA polymerase engineered for real-time PCR. KAPA SYBR® FAST DNA Polymerase has been evolved to perform optimally in stringent qPCR reaction conditions, exhibiting signifcant improve- ments in signal-to-noise ratio, cycle threshold (Ct), linearity, speed, and sensitivity.  KAPA SYBR® FAST Kits are supplied as a ready-to-use 2x Master  Mix containing all reaction components, except primers and template.  Kits are available for all qPCR instruments, including the Roche LightCycler® 480. [read more]

KAPA Blood PCR Mix
Eliminate the need for purifcation with the frst polymerase engineered for PCR direct from blood. The enzyme is available in KAPA Blood PCR Mix A or B, two optimized, easy-to-use 2x formats containing all PCR components except primers and template (whole blood). KAPA Blood PCR Mix is ideal for end-point PCR using one or more primer sets (including fuorescently labeled primers), end-point PCR followed by direct restriction enzyme digestion specifc for SNPs, and paternity testing using the Promega PowerPlex® 16 System.[read more]

  KAPAHiFi™ DNA Polymerase
A second-generation polymerase engineered for extreme fdelity and robustness. KAPAHiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fdelity polymerases and polymerase blends. The intrinsic high processivity of KAPAHiFi results in signifcant improvements in yield, speed, target length, and the ability to amplify difcult templates.  KAPAHiFi  exhibits over 30x increased fdelity as compared to wild-type Taq polymerase. [read more]
 

KAPA2G™ Fast HotStart
The ultimate hot start enzyme for extreme speed and performance. Evolved specifcally for speed and high performance, KAPA2G Fast HotStart ofers Fast PCR based on the intrinsic ability of the KAPA2G Fast DNA polymerase to synthesize DNA at a much faster rate than wild-type polymerases.  1 second per kilobase extension rate allows for increased productivity and faster time to results without sacrifcing performance [read more]

 

KAPALongRange™ DNA Polymerase
Engineered for long templates and extreme sensitivity. The KAPALongRange system is engineered for the amplifcation of long and complex targets up to 20kb.  The system is optimized specifcally for high yields from limiting starting DNA and exhibits extreme sensitivity.  KAPALongRange also exhibits a 4x improvement in fdelity as compared to standard Taq DNA polymerase.

[read more]