Ethidium bromide (EB) is most commonly used nucleic acid stain in molecular biology laboratories. It has been proved to be strong carcinogen and therefore considered hazardous for laboratory personnel and environment.

Midori Green DNA Stain is a nucleic acid stain which can be used as a safer alternative to the traditional Ethidium bromide stain for detecting nucleic acid in agarose gels. It is as sensitive as Ethidium bromide and can be used exactly the same way in agarose gel electrophoresis.

Alternative dye for ethidiumbromide

Figure 1 Agarose gels,stained by Midori Green DNA Stain (A) and Ethidium bromide (B).

Safety: Midori Green DNA Stain was tested in the mouse bone marrow micronucleus test en.wikipedia.org/wiki/Micronucleus to evaluate the genotoxic potential of this new dye. The result was negative ( in contrast to Ethidiumbromide and other dyes ) Furthermore the dye was applied to a chromosome aberration test en.wikipedia.org/wiki/Chromosome_abnormality. We can state that Midori Green is non-carcinogenic and according to the Ames test en.wikipedia.org/wiki/Ames_test it causes significantly fewer mutations than Ethidium bromide (Figure 2). If you like to get more information, please download the safety report.

less mutagenic as ethidiumbromide

Figure 2. Summary of Ames test results. Mutagenicity ratio of Midori Green DNA Stain and Ethidium bromide with metabolic activation (S9) in TA98 mutagenicity assay: a dose-response. According to the results of the Ames test, Midori Green DNA Stain is significantly less mutagenic compared to Ethidium bromide.

Protocol:

Prepare 100 ml of agarose gel solution (concentration from 0.8-3.0%) and heat until the solution is completely clear and no small floating particles are visible.
Add 2-5 µl of Midori Green DNA Stain to the gel solution and mix it gently.
Cool the gel to 50-60ºC and cast the gel, into the gel tray.
When the gel is solid, load the samples and perform electrophoresis.
Detect the bands under UV illuminator.

Notes: The thickness of gel should < 0.5cm. Repeated melting of gels containing Midori Green DNA Stain may result in low sensitivity. Midori Green DNA Stain is non-carcinogenic but may irritate skin and eyes. Please wear gloves while handling.

Disposal: Midori Green is not classified as hazardous waste, thus can be safely disposed of down the drain or as regular trash. Waste must be disposed of in accordance with federal, state and local environmental control regulations.

Ordering information:

	Cat.No.	Vol.
Midori Green	MG01	25 µl
Midori Green	MG02	1 ml

If you like to order the product give us a call (+49 2421 2084690) or send us an email mailto:info@nippongenetics.euand we send you a quote