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Go from cells to PCR in less than 10 mins! Ask for your free sample today
Nippon Genetics is proud to introduce a novel reagent specially formulated in order to eliminate nucleic acid purification for PCR analysis.
So far DNAreleasy has been tested on various cell types, including:
Human Buffy coat
Human fibroblasts tissue culture cells
Human sperm
Mouse & rat tissue culture cells
Mouse tail
Various plants (leaf, flower and flour): Arabidopsis, Cabbage, Oilseed Rape, Oats, Soya & Maize
Flea
Drosophila
Yeast
Gram +ve bacteria
Gram –ve bacteria
Viruses
Fungi
The protocol is quite simple and can be downloaded from this Web page dnareleasy/protocol/
A short version is available here:
Protocol:
| 1) | Mix cells with 20 µl of DNAreleasy. It is important that the lysis solution covers the cells completely. | |
| 2) | Overlay with mineral oil if necessary (for Thermal Cycler without heated lid) | |
| 3) | Place in a Thermal Cycler | |
| 4) | Lysis profile: Step 1: 75°C for 5 mins Step 2: 96°C for 2 mins Step 3: 20°C hold |
After lyis part of all of the DNAreleasy lysate can be used directly in PCR. DNAreleasy can make up on to 40% of most PCR mixes. Or it can be stored at -20°C for future use.
(Note: DNAreleasy is a complex solution which releases (does not purify) the DNA from cells. An accurate estimation of DNA yield after lysis is therefore difficult and can not be done using a spectrophotometer)
DNAreleasy Kit 50 preparation; Cat. No.: LS02
DNAreleasy Kit sample kit 10 preparation; Cat. No.: LS01
Store DNAreleasy at +4°C FOR RESEARCH USE ONLY